<form id="r1z7z"></form>
      <noframes id="r1z7z">
      <noframes id="r1z7z"><span id="r1z7z"></span>
      <form id="r1z7z"></form>
      <address id="r1z7z"></address>

        

        <address id="r1z7z"><address id="r1z7z"><nobr id="r1z7z"></nobr></address></address>
        










        
	
        
		
		
        服務熱線： 
        15821734033
        		
		
        
	
        

        




        
	
	
	
        

        














        




        
	
	
        
		
        
  
		
		
	
        
	
	
	
        
	
		

		
		
        
			產品展示
        您現在的位置：首頁  >  產品展示  >    >  常規ELISA試劑盒  >  D1045 5-Hydroxymethyl dCTP
        
		
        

		
        
			
        
				
        

				
        
					
        
						
        
							
        
						
        
					
        
					
        
				
        

				
        
			
        
			

			
        
				
        D1045 5-Hydroxymethyl dCTP
        
				
          
					
        • 產品型號：
          • 
					
        • 產品時間：2024-08-09
          • 
					
        • 簡要描述：D1045 5-Hydroxymethyl dCTP   上海慧穎生物優勢供應，*，！
             上?；鄯f生物，值得您信賴的選擇??！
          • 
				
        
				
			
        
			
        
		
        

		
        
			
          
				
        • 產品簡介
          • 
			
        
		
        

		
        
			
        
				
        D1045 5-Hydroxymethyl dCTP   
        品牌：ZYMO Research
        代理商：慧穎生物
        庫存：貨期產品
        D1045 5-Hydroxymethyl dCTP   
        Ready to use 5-Hydroxymethylcytosine mix (dATP, dTTP, dGTP, d5hmCTP) and 5-Methylcytosine dNTP mix (dATP, dTTP, dGTP, d5mCTP) is of ultra high purity; >99% trisphosphate by HPLC.
        Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases.
        Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.
        About Hydroxy/Methylated Nucleotides
        5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
         For 5-hmCFor 5-mC
        ReagentVolumeFinal ConcentrationVolumeFinal Concentration
        2X Reaction Buffer25 µl1X25 µl1X
        dNTP Mix0.5 µl0.25 mM each dNTP0.5 µl0.25 mM each dNTP
        Forward Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
        Reverse Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
        Template DNAVariable< 200 ng/50 µlVariable< 200 ng/50 µl
        ZymoTaq™ DNA Polymerase (5 U/µl)1 µl5 U/50 µl0.4 µl<2 U/50 µl
        ddH20to 50 µl-to 50 µl-
        Total Volume50 µl 50 µl 
         
        Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.
        Specifications
        Concentration5-Hydroxymethylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5hmCTP). 
        5-Hydroxymethyl dCTP: 10µmol/100 µl as a [100 mM] solution.
        5-Methylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5mCTP).
        5-Methyl dCTP: 1µmol/100 µl as a [10 mM] solution.
        ApplicationsPCR amplicons containing 5-(hydroxy)methylcytosine are useful in a variety of applications such as: 
        • The study of 5-(hydroxy)methylcytosine binding/interacting protein
        • Define methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine.
        Storage5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
        
			
        
		
        
		
		

		
		
        
			



        

        
	
        留言框  
        
	
          
		
        • 
			
          產品：
          
			
          
		
          • 
		
        • 
			
          您的單位：
          
			
          
		
          • 
		
        • 
			
          您的姓名：
          
			
          
		
          • 
		
        • 
			
          聯系電話：
          
			
          
		
          • 
		
        • 
			
          常用郵箱：
          
			
          
		
          • 
        
        • 
			
          省份：
          
			
          
		
          • 
		
        • 
			
          詳細地址：
          
			
          
		
          • 
		
        
        • 
			
          補充說明：
          
			
          
		
          • 
		
        • 
			
          驗證碼：
          
			
          
            	
          
                    
                    
                
          
                請輸入計算結果（填寫阿拉伯數字），如：三加四=7
            
          
		
          • 
		
        • 
			
          
			
          
		
          • 
	
        	
    

        

        

		
        

		
	
	
        
	

        





        
	
        
		
        
			 上海慧穎生物科技有限公司 版權所有    備案號：滬ICP備12016933號-2
			
        技術支持：化工儀器網    管理登陸    網站地圖
        

		
        
	
        

        



        
	
        
		
		
		
		
		
		
        
			
			
        
				
        
					
					
        
						
        聯系電話：
         15821734033
        
					
        
				
        
				
        
			
        
		
        
		
        
			
			
        
				
        
				
        微信服務號
        
			
        
			
        
		
        
	
        

        
	


        
	

        



 
  





午夜伦理宅宅235